Protein-protein interaction map for yeast TFIID
نویسندگان
چکیده
منابع مشابه
A protein-protein interaction map of yeast RNA polymerase III.
The structure of the yeast RNA polymerase (pol) III was investigated by exhaustive two-hybrid screening using a library of random genomic fragments fused to the Gal4 activation domain. This procedure allowed us to identify contacts between individual polypeptides, localize the contact domains, and deduce a protein-protein interaction map of the multisubunit enzyme. In all but one case, pol III ...
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SETH4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of Arabidopsis thaliana. This fragment was PCR amplified using Kod Hi Fi DNA polymerase enzyme. This fragment was cloned into pGBKT7 bate vector and transformed E. coli DH5? cells containing vector were selected on LB medium containing Kanamycin. Finally, pGBKT7-SETH4 bate was transformed into yeast ...
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General transcription factor TFIID, consisting of TATA-binding protein (TBP) and TBP-associated factors (TAFs), plays a central role in both positive and negative regulation of transcription. The TAF N-terminal domain (TAND) of TAF1 has been shown to interact with TBP and to modulate the interaction of TBP with the TATA box, which is required for transcriptional initiation and activation of TAT...
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As protein-protein interaction plays a major role in most cellular processes, identifying the full repertoire of interacting protein pairs in the cell is of great importance. This challenge has been addressed both experimentally and computationally. Large-scale experimental studies provide data sets of interacting proteins. These data sets, while only partial and noisy, allow us to characterize...
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The availability of complete genome sequences of numerous model organisms has initiated the development of new approaches in biological research to complement conventional biochemistry and genetics. Consequently, high-throughput methodologies also need to be applied in the emerging field of proteomics. Here, we discuss several methods that have been developed in the past years in order to chara...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2003
ISSN: 1362-4962
DOI: 10.1093/nar/gkg204